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Patterning of renal medullary vasa recta bundles takes place in a narrow developmental window in rats and humans and is dependent on Angiotensin II AT1 receptors
Author(s) -
Tinning Anne,
Jensen Boye,
Johnsen Iben,
Chen Daian,
Coffman Tom,
Madsen Kirsten
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.796.1
Subject(s) - vasa recta , kidney development , nephron , angiotensin ii , kidney , biology , glomerulus , endocrinology , receptor , angiotensin ii receptor type 1 , medullary cavity , medicine , anatomy , renal medulla , gene , embryonic stem cell , biochemistry
BACKGROUND Nephron segments of the renal medulla differentiate and expand during late stages of kidney development. Patterning of vasa recta bundles and establishment of normal tubulo‐vascular organization is not fully understood. We hypothesize that patterning of vasa recta bundles takes place during late developmental stages and is dependent on angiotensin II (ANGII) signaling. METHODS & RESULTS: Endothelial CD31‐staining of rat kidney tissue showed no vasa recta bundles as late as postnatal (P) day 7. At P10, first vascular assemblies appeared with subsequent expansion to regular bundles size at P21. Adult ANGII AT1A/1B receptor knockout mice displayed aberrant, poorly defined vasa recta bundles with interspersed thick ascending limbs of Henles loop. Human fetal kidney tissue was obtained from autopsies at gestational ages (GA) 23 to 42. Single, parallel postglomerular microvessels was defined at GA 23, and first signs of bundle formation appeared by the beginning of the third trimester (GA 27‐28). SUMMARY: 1) Patterning of vasa recta bundles takes place between P7 and P10 in rats and by GA 27‐28 in humans. 2) Genetic deficiency of ANGII AT1 receptors leads to disturbed patterning of vasa recta bundles. CONCLUSION Renal medullary tubulo‐vascular organization is established in a narrow developmental window in rats and humans and is in mice dependent on ANGII‐AT1 receptors. FUNDING: Novo Nordisk & Novozymes A/S, The Danish Heart Association, The Region of Southern Denmark.