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Effect of Electrospun Scaffold Fiber Alignment on Platelet Functions
Author(s) -
Han Jooli,
Soaita Ioana,
O'Hara Joseph,
Yin Wei,
Rubenstein David
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.792.1
Subject(s) - electrospinning , scaffold , platelet , chemistry , platelet activation , tissue engineering , nanofiber , biomedical engineering , adhesion , biophysics , nanotechnology , materials science , immunology , medicine , organic chemistry , polymer , biology
Electrospinning is an efficient process for the fabrication of microvascular tissue engineering scaffolds. It has recently become evident that platelets can facilitate microvascular network growth by acting as a bridging cell between neighboring endothelial cells. However, if platelet thrombotic activities are enhanced through direct/indirect contact with the scaffolds, microvascular network growth is hindered. Thus, the objective of this work was to determine the effect of electrospun scaffold fiber alignment on platelet functions, to determine whether or not these scaffolds are suitable for accelerated microvascular tissue engineering applications. We hypothesized that these scaffolds would not elicit platelet responses. Electrospun scaffolds were fabricated from combinations of cellulose acetate, chitosan and/or poly‐caprolactone, using traditional and rotating cylinder electrospinning. A time course for platelet activation, adhesion potential and aggregation was obtained for up to 4 hours after incubation with scaffolds. Imaging flow cytometry and optical platelet aggregometry, antagonized with the thrombin receptor antagonist or collagen, were used to analyze platelet functional changes. Our results show that in general, all of these scaffolds are compatible with platelets; they do not enhance platelet activation, adhesion or aggregation responses, as compared to platelet samples not exposed to scaffold. Scaffold alignment did not alter platelet functions, except for some formulations consisting of PCL. The duration of incubation with scaffolds also did not alter platelet responses. These data suggest that these novel aligned or random scaffolds can be used in microvascular tissue engineering applications. Thanks to the NIH.