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Sphingosine‐1‐Phosphate (S1P) Maintains Normal Microvascular Permeability by Preserving Endothelial Surface Glycocalyx (ESG)
Author(s) -
Zhang Lin,
Zeng Min,
Fan Jie,
Curry FitzRoy,
Tarbell John,
Fu Bingmei
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.791.7
Subject(s) - glycocalyx , chemistry , anatomy , mesentery , perfusion , vascular permeability , biophysics , biology , biochemistry , medicine , endocrinology
To test the hypothesis that the plasma phospholipid S1P contributes to the maintenance of normal vascular permeability by protecting the ESG of blood vessels, we quantified the ESG in post‐capillary venules of rat mesentery by immunostaining the heparan sulfate (HS) in the presence and absence of S1P. HS is the most abundant component of the glycosaminoglycans of vessels. Rats (SD, 250‐300g) were anesthetized with pentobarbital sodium given subcutaneously and kept warm on a heating pad. A midline incision (~2 inch) was made in the abdominal wall and the mesentery was gently taken out from the abdominal cavity and arranged on the surface of a glass coverslip for the measurement. A 25‐30µm diameter artery connecting a group of downstream microvessels was cannulated by a theta micropipette. After 20 min perfusing with 1% fatty acid free bovine serum albumin with and without 1 µM S1P through one lumen of the micropipette, the perfusion was switched to that additionally containing 20 µg/ml FITC‐anti‐HS and lasted for ~2.5h under 4 0 C superfusate. After washing away the free dye, images of vessels with labeled FITC‐anti‐HS were collected and quantified by NIH Image J. In the absence of S1P, the intensity of FITC‐anti‐HS was only ~11% of that in the presence of S1P (n=3, p<0.001). The result is in consistent with in vitro measurements (Zeng et al, Am J Physiol, 2014 ) and conforms to the hypothesis. Supported by NIH R01HL094889 and SC1CA153325.