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Tetra‐triethyleneoxysulfonyl substituted zinc phthalocyanines are promising new photosensibilisators for cancer treatment with Photodynamic Therapy (PDT)
Author(s) -
Kuzyniak Weronika,
Ermilov Eugeny,
Atilla Devrim,
Gürek Ayse,
Nitzsche Bianca,
Derkow Katja,
Hoffmann Björn,
Steinemann Gustav,
Pries Axel,
Ahsen Vefa,
Höpfner Michael
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.785.4
Subject(s) - photodynamic therapy , phototoxicity , photosensitizer , singlet oxygen , in vivo , cancer research , chemistry , apoptosis , cancer cell , cancer , photochemistry , medicine , in vitro , biochemistry , biology , oxygen , organic chemistry , microbiology and biotechnology
PDT has emerged as an effective and minimally invasive treatment option for several diseases, including some forms of cancer. However, several drawbacks of the approved photosensitizers (PS) hampered the clinical effectiveness of PDT so far. Phthalocyanines (Pc) are interesting PS with a strong light absorption in the favourable red spectral region and a high quantum yield of cancer cell destroying singlet oxygen generation. Here, we evaluated the suitability of tetra‐triethyleneoxysulfonyl substituted zinc phthalocyanines (ZnPc) as novel PS for PDT. ZnPc‐induced phototoxicity was studied in pancreatic carcinoid (BON) and squamous esophageal cancer cells (Kyse‐70). Photoactivation (10 J /cm²) of ZnPc‐loaded cells (1‐10µM) revealed strong phototoxic effects, leading to a dose‐dependent decrease of BON and Kyse‐70 cell proliferation of up to almost 100%, the induction of apoptosis and a G1‐phase arrest of the cell cycle. By contrast, ZnPc‐treatment without illumination did not induce any cytotoxicity, apoptosis, cell cycle arrest or decreased cell growth. Antiangiogenic effects of ZnPc‐PDT were investigated in vivo by performing CAM assays, which revealed a marked degradation of blood vessels and the capillary plexus of the chorioallantoic membrane. Our study shows the extraordinary potential of the novel ZnPc photosensitizer for PDT‐based cancer treatment.