Premium
The Role of Caveolin‐1 in 5‐Hydroxytryptamine Receptors Mediated Epidermal Growth Factor Receptor Transactivation In Vascular Smooth Muscle
Author(s) -
Guner Sahika,
Akhayeva Tamila,
Gurdal Hakan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.782.3
Subject(s) - transactivation , vascular smooth muscle , transfection , receptor , agonist , caveolin 1 , epidermal growth factor receptor , epidermal growth factor , microbiology and biotechnology , cancer research , phosphorylation , chemistry , endocrinology , medicine , biology , biochemistry , smooth muscle , transcription factor , gene
5‐Hydroxytryptamine (5‐HT) receptors have numerous functions in vascular and central nervous system. We have previously shown that 5‐HT mediated EGF (epidermal growth factor) receptor transactivation contributes to vascular contractility. In this study, we investigated the roles of caveolin‐1 in 5‐HT receptor mediated EGFR transactivation and contraction. We evaluated EGFR transactivation and contractile responses produced by 5‐HT in β‐cyclodextrin (β‐CD) treated and untreated rat aorta. 5‐HT, selective 5HT 2A receptor agonist α‐Methyl‐5HT and selective 5HT 1B/D receptor agonist sumatriptan stimulated EGFR phosphorylation (pEGFR) were also examine in caveolin‐1 siRNA (si‐Cav‐1) transfected A7R5 vascular smooth muscle cell lines. β‐CD treatment or AG1478 decreased maximal contractile responses of 5‐HT and 5‐HT mediated EGFR phosphorylation in rat aorta and A7R5 cell lines. Furthermore, 5‐HT and α‐Methyl‐5HT mediated EGFR phosphorylation were inhibited by transfection of si‐Cav‐1 to A7R5 cells. However, selective 5HT 1B/D receptor mediated phosphorylation of EGFR did not change in si‐Cav‐1 transfected A7R5 cells. Our results showed that caveolin‐1 play significant role in 5HT 2A receptor, but not 5HT 1B/D receptor mediated EGFR transactivation.