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Apoptosis activation under the action of immunomodulators on the course of experimental myocardial infarction
Author(s) -
Sarapultsev Alexey,
Chupakhin Oleg,
Sarapultsev Petr,
Sidorova Larisa
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.781.5
Subject(s) - apoptosis , fas receptor , immunohistochemistry , myocardial infarction , immune system , medicine , programmed cell death , chemistry , immunology , cancer research , biochemistry
The goal of the study was the estimation of the apoptosis's processes activation during experimental myocardial infarction. Immunohistochemical study was performed on paraffin sections of 5‐6 microns thick with the indirect Coombs test. The number of cells expressing CD95 and P53 were evaluated. The experiment was carried out on 45 male albino rats with EMI. The immune modulators used in test groups were 5‐phenyl‐6H‐1,3,4‐thiadiazin‐2‐amine's compound and 3‐aminophtalhydrazine. According to the results of virtual screening (via computer programs PASS/GUSAR) these compounds are able to induce apoptosis. Activation of apoptosis was estimated by the expression of CD 95 and P53 markers via indirect immunohistochemical method. Under the action of test compounds, the significant activation of extrinsic (CD95) and intrinsic (P53) apoptosis pathways was observed already on the first day of experiment. Increased expression of CD95 was observed from the 1 to 14 days of experiment. Statistically significant increase in expression of P53 was revealed on 7 and 14 days. According to the histological data, that activation of apoptosis under the action of compounds was associated with more favorable histological picture. Thus, cell death by apoptosis is more conducive to further healing of myocardial infarction.