Premium
The Methanol Extract of Garcinia kola Seed Blunts Lipopolyssacharide (LPS)‐ and Angiotensin II‐induced Cell Proliferation as well as Nitric Oxide Production in In Vitro Vascular Smooth Muscle Cells (VSMC) Assay
Author(s) -
Adedapo Adeolu,
Omobowale Temidayo,
Oyagbemi Ademola,
Yakubu Momoh,
Oyekan Adebayo
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.773.6
Subject(s) - vascular smooth muscle , angiotensin ii , nitric oxide , garcinia kola , in vitro , chemistry , smooth muscle , medicine , cell , endocrinology , biology , traditional medicine , biochemistry , blood pressure
The methanol extract of the seed of Garcinia kola also known as “bitter kola” was assessed for its antiproliferative potential on the Vascular Smooth Muscle Cells (VSMCs) in in vitro study. Using lipopolyssacharide (LPS) at 100µg/ml, 1 nm of Angiotensin II (10 ‐8 ) and 1 µM of Angiotensin II (10 ‐6 ) as mitogens, induction of cell proliferation was embarked upon while MTT assay was used to measure cell viability. In this study, 100µg/ml of LPS was also used to induce nitric oxide production. The use of Angiotensin II (10 ‐8 ), Angiotensin II (10 ‐6 ) and LPS in the treatment of VSMCs led to significantly enhanced proliferation of these cells. In the similar vein, LPS caused significant (p<0.05) increase in nitric oxide (NO) production when treated with VSMCs. Treatment with GK (25, 50 & 100 µg/ml) blocked cell proliferation in a dose‐and time‐dependent fashion and this was also followed by significantly reduced excessive NO production due to treatment with this extract at these doses. The results obtained from this study may be due to the anti‐oxidant and anti‐inflammatory properties of this extract.