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Identification of a New Sesquiterpine Component from Petasites japonicus Leaves and Its Anti‐asthma Effect
Author(s) -
Im DongSoon,
Kang Saeromi,
Kim JungMin,
Choi YoungWhan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.773.5
Subject(s) - degranulation , nitric oxide , chemistry , pharmacology , nitric oxide synthase , antigen , traditional medicine , immunology , biology , biochemistry , medicine , receptor , organic chemistry
Background Petasites genus has been known to have anti‐allergic and anti‐asthma effects. Lipophilic extracts of rhizomes of Petasites hybridus L. (the butterbur) have been used in treatment of asthma in the West, while the leaves of Petasites japonicus are eaten as a food in Korea. In order to identify the anti‐allergic and anti‐inflammatory active ingredients, we studied the effects of several compounds isolated from Petasites japonicus leaves (a butterbur species). METHODS: MurWeSHL10 was isolated from Petasites japonicus leaves. By measuring beta‐hexosamidase activity, antigen‐induced degranulation was measured in RBL‐2H3 mast cells. In peritoneal macrophages, induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX‐2) was measured by Western blotting. RESULTS: MurWeSHL10, a major component of the Petasites japonicus leaves, was isolated and its structure was identified as a bakkenolide‐type sesquiterpine. MurWeSHL10 inhibited the antigen‐induced degranulation of beta‐hexosamidase in RBL‐2H3 mast cells in a concentration‐dependent manner. However, MurWeSHL10 did not inhibit antigen‐induced Ca 2+ increase in RBL‐2H3 cells. Furthermore, MurWeSHL10 inhibited the lipopolysacharide‐induced induction of iNOS significantly, but slightly inhibited COX‐2 induction in mouse peritoneal macrophages. Nitric oxide production was also inhibited by MurWeSHL10 in the macrophages. CONCLUSION MurWeSHL10 has anti‐allergic and anti‐inflammatory effects and may be utilized as a potent agent for the treatment of asthma.