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Effect of chitosan film containing carvacrol , a phenolic monoterpene, on wound healing in rats
Author(s) -
Barreto Rosana,
AlbuquerqueJunior Ricardo Luiz,
PereiraFilho Rose Nely,
Lima Pollyana,
Nunes Rogeria,
Barreto Andre,
Lucca Waldecy,
Camargo Enilton,
QuintansJunior Lucindo
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.773.4
Subject(s) - carvacrol , chitosan , granulation tissue , wound healing , essential oil , chemistry , edema , monoterpene , traditional medicine , pharmacology , food science , medicine , surgery , biochemistry
Carvacrol, a phenolic monoterpene present in the oregano essential oil. The aim of this study was to investigate the wound healing activity of carvacrol incorporated to chitosan bioactive films on excision wound model in rats. Wistar rats, weighing 250‐300 g, were anesthetized and an excisional wound was performed. Rats were treated with chitosan bioactive film; chitosan bioactive film containg carvacrol 0.5% or 1%, for 3, 7, 14 or 21 days. The residual wounds were analyzed and removed. Serial histological sections were obtained and stained by histochemical techniques (Hematoxilin/Eosin and Sirius red). Experimental protocols were approved by the Animal Care and Use Committee at the UFS/Brazil. Carvacrol incorporated to chitosan films reduced wound areas (p < 0.01) and tissue edema (p < 0.001), induced earlier granulation tissue formation, increased cell proliferation, increased ephitelization rates (p < 0.01) and improved collagenization when compared to the control group. It is suggested that carvacrol incorporated to chitosan films has a potential use on skin wound healing of rats. Financial support: FAPITEC/SE/Brazil; CNPq/Brazil and CAPES/Brazil.