Premium
Curcumin Suppresses Human Circulating Fiobrocytes Differentiation and Migration by Regulating CCR7 and CCL21 Expression
Author(s) -
Li Yu,
Fu Xuyan,
Zhang Dongwei,
Li Yadong,
Niu Jianzhao
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.773.14
Subject(s) - curcumin , fibrocyte , microbiology and biotechnology , chemistry , flow cytometry , in vitro , biology , immunology , biochemistry , anatomy
Recent studies have confirmed that circulating fiobrocytes may play important roles in the formation and development of fibrosis. Curcumin, a polyphenolic compound isolated from turmeric (Zingiberaceae), has been shown to have anti‐fibrosis effects in various organs. Here, we illustrated curcumin's effect on modulating the viability, differentiation and migration of human circulating fiobrocytes. Following the isolation and purification by density gradient centrifugation, cells were identified to be positive of COL I, CD45 and CCR7 by flow cytometry and immunofluorescence. Curcumin treatment (72 h; 20 μmol•L ‐1 ) significantly reduced cells propagation, the levels of COL I, α‐SMA and CCR7,as well as the TGF‐βl secretion in human circulating fibrocytes. The possible mechanism of curcumin on blocking the differentiation and migration of human circulating fibrocytes is through regulating CCR7/CCL21 signaling pathway, in particular by reducing CCR7 expression. These effects of curcumin on human circulating fibrocytes may contribute to resolving the fibrosis, which is the first report to describe the effect of curcumin on human circulating fibrocytes in vitro.