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Morphine Induced Changes of Gene Expression in the Differentiated SH‐SY5Y Human Neuroblastoma Cell
Author(s) -
Randell Kesa,
Goodman Carl
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.768.9
Subject(s) - morphine , sh sy5y , opioid , gene expression , medicine , analgesic , transcription factor , bioinformatics , pharmacology , gene , biology , neuroblastoma , cell culture , genetics , receptor
PURPOSE: Scientific advances in various areas have been made however; morphine remains the leading opioid analgesic of choice for the treatment of moderate to severe pain. Although morphine has very potent analgesic actions, it has adverse side effects including the development of tolerance resulting in its limited use. The underlying mechanism of opioid tolerance is unclear, but focus in recent years has shifted to the induction of transcription factors. The present study aims to gain a better understanding of alterations in gene expression induced following opioid treatment using real‐time quantitative PCR. METHODS: The expression of 91 transcriptional factor genes using the differentiated SH‐SY5Y human neuroblastoma cell line as a model, with BioRad Prime PCR Pathway Plates TM analyzed with real‐time quantitative PCR to identify the major target genes regulated by chronic morphine treatment. EXPECTED RESULTS: Previous opioid tolerance studies suggest significant changes will be noted in CREB1 and FOSB gene expression levels following chronic (24 hrs) morphine treatment (10 μM). Based on these results, in the current study, we expect to confirm the results of previous studies and add new targets to further research the development of opioid tolerance. DISCUSSION Overall results from this study will provide an understanding on the transcriptional mechanisms involved in the development of morphine tolerance.