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Expression of non‐collagenous proteins in facial tissue of children with cleft lip and palate
Author(s) -
Smane Liene,
Pilmane Mara,
Akota Ilze
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.765.2
Subject(s) - osteopontin , osteoprotegerin , medicine , immunohistochemistry , ossification , rankl , osteoclast , bone remodeling , bone tissue , pathology , anatomy , activator (genetics) , receptor
Objective Cleft lip and palate (CLP) is a congenital anomaly characterized by the inappropriate fusion of the upper lip, alveolus, and secondary palate. This study investigated whether expression of osteoprotegerin (OPG), osteopontin (OPN), andosteocalcin (OC), which are essential for the normal bone remodeling and ossification, is dysregulated in children with CLP. Design: Bone tissue samples were obtained from patients with complete bilateral (CB) CLP (n = 14) during corrective plastic surgery and unaffected control subjects (n = 9). OPN, OC, and OPG expression was assessed by immunohistochemistry, and data were analyzed with the Mann‐Whitney test. Results: In patients, OPG immunoreactive osteocytes were seen in low to moderate number, but the overall number of OPG‐positive bone cells was lower than that in controls (z = −2.25; P = 0.025). OPN expression was observed only in sporadic cells in the bone tissue of 3 patients, in contrast to the control group (z = −2.96; P = 0.003). OC‐positive osteocytes were present in moderate to high numbers in patients with CBCLP and in controls. There was no significant difference in mean between the study groups (z = −1.36; P = 0.175). Conclusions The prominent expression of OC characteristic for CBCLP affected hard tissue indicates a high potencial of bone mineralization. Few OPG‐positive osteocytes in the bone tissue implicate the dysregulation of osteoclast differentiation, maturation, and activity, but few OPN‐containing cells prove the common dysregulation of bone remodelation in clefts. This study was funded by the Support for Doctoral and Post‐doctoral Investigations Riga Stradins University fellowship.