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Growth Hormone Administration Influence on Soleus Muscle of Heart Failure Rats
Author(s) -
Lima Aline,
Damatto Ricardo,
Pagan Luana,
Gomes Mariana,
Bonomo Camila,
Cezar Marcelo,
Damatto Felipe,
Zanati Silmeia,
Okoshi Katashi,
Okoshi Marina
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.764.6
Subject(s) - soleus muscle , growth hormone , endocrinology , medicine , hormone , administration (probate law) , heart failure , skeletal muscle , political science , law
Objective: To evaluate the effects of growth hormone (GH) on satellite cells activation and expression of trophism‐related proteins in soleus muscle of rats with aortic stenosis (AS)‐induced heart failure. Methods: Male Wistar rats were subjected to thoracotomy for ascending AS induction. After developing tachypnea, rats were assigned into three groups (n=8): Sham, AS and AS treated with GH (AS‐GH, 2 mg/kg/day for 14 days). Echocardiogram was performed before and after treatment. Muscle trophism was evaluated by histologic analysis. Protein expression was analyzed by Western‐blot and satellite cells activation by immunofluorescence. Statistical analysis: Anova and Tukey or Kruskal‐Wallis and Student‐Newman‐Keuls. Results: Before treatment, cardiac variables did not differ between AS and AS‐GH groups. GH administration preserved body weight (Sham 492±32; AS 420±32; AS‐GH 486±69 g; p<0.05 AS vs Sham and AS‐GH). Left ventricular posterior wall thickness and right ventricle weight/body weight ratio were higher in AS and AS‐GH than Sham. Soleus cross‐sectional area was smaller in both AS groups than Sham (Sham 3,556±447; AS 2,882±422; AS‐GH 2,868±591 µm 2 ; p=0.02). IGF‐1 expression was higher in AS‐GH (Sham 1.0±0.3; AS 1.0±0.5; AS‐GH 1.7±0.5 arbitrary units; p=0.004). p‐PI3K/GAPDH was higher in AS and AS‐GH than Sham and total PI3K [Sham 1.0 (0.6‐1.3); AS 1.3 (1.0‐2.5); AS‐GH 2.5 (2.0‐6.0) arbitrary units; p=0.04] was higher in AS‐GH than Sham. MyoD, MRF4, myogenin, myostatin, follistatin, akt, atrogin‐1, MuRF‐1 and Pax‐7 protein levels did not differ between groups. Satellite cells activation was increased in both AS groups. Conclusion Growth hormone administration increases IGF‐1 and PI3K intracellular signaling in the soleus muscle of heart failure rats.