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mir‐548n Expression in HEPG2 and HEP2 cells Is Affected By The Culture Medium Zinc Concentration
Author(s) -
Grider Arthur,
Lewis Richard,
Laing Emma,
Bakre Abhijeet,
Tripp Ralph
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.761.5
Subject(s) - zinc , chemistry , expression (computer science) , microbiology and biotechnology , biology , computer science , organic chemistry , programming language
The objective of this research was to study the impact of zinc depletion / supplementation on plasma microRNAs, which may be then used as biomarkers for zinc status. Clinical data from plasma from adolescent females on zinc supplementation (9 mg elemental Zn / wk for 4 weeks) exhibited changes in exosomal microRNAs of which mir‐548n, showed a 57% increase. Hepatocyte (HEPG2) and lung epithelium (HEp2) cells were grown in normal (15μM), zinc depleted (Chelex‐100 treated) or zinc supplemented (50μM) media for 48 hr to validate the clinical data. HEPG2 cells grown in 15 μM Zn vs. 0 μM Zn showed a 10‐fold increase in miR‐548n and remained at that level in cells grown in 50 μM Zn. In the HEp2 cells, mir‐548n expression decreased 3‐fold in cells grown in 15 μM Zn vs. 0 μM Zn, and increased 7‐fold in cells grown in 50 μM Zn vs. 15 μM Zn. These data indicate that mir‐548n expression is regulated by Zn. Its secretion into plasma exosomes isolated from human subjects may be indicative of its increased expression in tissues in response to Zn supplementation