Premium
Modulation of Intestinal Microbiota by Dietary Selenium and 15kDa Selenoprotein Expression in Inflammatory Colon Cancer
Author(s) -
Canter Jessica,
Sheckells Sarah,
Saylor Charlotte,
Patterson Angelica,
Carlson Bradley,
Gladyshev Vadim,
Yu Yunkai,
Cao Liang,
May Meghan,
Davis Cindy,
Hatfield Dolph,
Tsuji Petra
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.759.7
Subject(s) - azoxymethane , firmicutes , selenoprotein , aberrant crypt foci , biology , gut flora , carcinogenesis , microbiology and biotechnology , colorectal cancer , cancer , biochemistry , genetics , 16s ribosomal rna , oxidative stress , gene , superoxide dismutase , colonic disease , glutathione peroxidase
Selenium (Se) exerts its mechanistic effects through incorporation into selenoproteins. Many selenoproteins, such as the 15kDa selenoprotein (Sep15), are involved in cancer prevention and promotion. Sep15 ‐/‐ mice were shown previously to be resistant to chemically induced aberrant crypt foci. In a model of inflammatory colon tumorigenesis, Sep15 ‐/‐ mice formed a similar number and mass of tumors as controls when Se‐replete. However, under Se‐deficient conditions, lack of Sep15 protected against tumor formation. We investigated the contributions of dietary Se and Sep15 genotype on the composition of the gut microbiota using bacterial 16S rRNA, extracted from mouse fecal samples. Firmicutes, Proteobacteria and Bacterioidetes were the main phyla represented in all mice. Interestingly, under Se‐deficient conditions, mice lacking Sep15 had a lower bacterial diversity than controls. That difference disappeared when mice were exposed to azoxymethane/dextran sulfate sodium to induce tumorigenesis. Serum inflammatory biomarkers and bacterial diversity are currently examined in mice on sufficient and supplemental Se diets. Funding:NCI Intramural support; NIH CA080946 (VNG); Office of Dietary Supplements; Towson University Jess & Mildred Fisher Endowed Chair (PAT)