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Effect of Long‐Term Dietary Selenium Deprivation and Aging on Selenoprotein Transcriptome in Short Telomere Mice
Author(s) -
Cao Lei,
Wu Ryan,
Cheng WenHsing
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.759.13
Subject(s) - selenoprotein , selenoprotein p , endocrinology , medicine , weanling , biology , gpx1 , selenium deficiency , senescence , selenium , chemistry , oxidative stress , catalase , organic chemistry , glutathione peroxidase
Mice or rats fed a diet deficient in selenium (Se) show changes of selenoprotein expression in a tissue‐ and selenoprotein‐specific manner. The objective of this study was to determine the effect of long‐term dietary Se deprivation and aging on selenotranscriptome in older mice. Weanling male Terc ‐/‐ premature aging mice were fed a Se‐deficient diet or the diet supplemented with Se (0.15 ppm as selenate) until they were sacrificed at 18 and 24 months of age. The mRNAs for selenoproteins were normalized with that for ß‐actin. Results from quantitative RT‐PCR analyses demonstrated that dietary Se deficiency decreased levels of mRNAs for Selh, Sepw1, and Gpx1 in the heart, lung, and/or pancreas. Interestingly, mRNA levels for several selenoproteins were increased by age in the testes of Se‐deprived mice. There were age‐dependent declines in the mRNA levels for Txnrd1 and Sepw1 in the heart, Sephs2 in the lung, and several selenoproteins in the pancreas. Taken together, selenoprotein mRNA expressions are regulated differentially by dietary Se deprivation, old age, or both in an organ‐ and selenoprotein‐specific manner in the Terc ‐/‐ mice displaying many features of age‐related degenerations.