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Effect of Prolonged Consumption of Sweeteners in CD1 Mice in Lymphocyte Populations from Different Lymphoid Organs.
Author(s) -
MartinezCarrillo Beatriz,
EscotoHerrera Jorge,
ValdésRamos Roxana,
JarilloLuna Rosa,
Benitez Alejandra
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.756.2
Subject(s) - sucralose , lymphocyte , spleen , endocrinology , medicine , chemistry , sucrose , lymphatic system , biology , immunology , food science
Intake of sweeteners from many products to reduce the energy is high, but the consumption for a long time and their effect on lymphocytes percentage has been scarcely studied. The aim of this study was to evaluate the effect of prolonged use of sweeteners in CD1 mice on lymphocyte populations from different lymphoid organs. We used 32 21‐day old CD1 mice, divided into 4 groups: Control (CL), Sucrose (SAC), Sucralose (SUC), Stevia (ST). Ten grams of each sweetener were diluted in 240mL of water, mice drank and fed ad libitum. Peripheral blood glucose, BMI and caloric index was quantified. Weight increased significantly (F=2.587, p<0.029), feed intake (F=16.40, p<0.001) and water consumption (F=21.65, p<0.001) caused mainly by SUC. No differences in final BMI (F=2.02, p<0.134) were found. The caloric index showed differences between groups (F=16.40, p<0.001), it was significantly elevated between CL and SAC group (p<0.001). The blood glucose concentration decreased significantly (F=5.155, p<0.006) in the EST group and increased in the SAC and SUC groups. Lymphocyte percentages decreased in whole blood (F=31.75, p<0.001), increased in spleen (F=7.93, p<0.001) and decreased in Peyer's patches, in all cases at the expense of SUC and EST (F=3.5, p<0.028). The percentage of lymphocytes decreased in Peyer´s patches in SUC and EST groups, indicating a local effect of these sweeteners in the intestinal mucosa.