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Protective effect of Aplysin on IKK/NF‐kB signaling pathway and related cytokines in rats exposed to ethanol
Author(s) -
Wang Wencheng,
Ge Na,
Ma Aiguo,
Liu Man,
Liang Hui
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.755.18
Subject(s) - ethanol , western blot , chemistry , proinflammatory cytokine , endocrinology , medicine , tumor necrosis factor alpha , nf κb , iκb kinase , signal transduction , pharmacology , inflammation , biochemistry , gene
This study is aim to evaluate the protective effect of Aplysin on IKK/NF‐kB signaling pathway and related cytokines in rats exposed to ethanol. Fifty male rats were randomly divided into five groups (control group, ethanol‐model group, low‐, medium‐, high‐ dose of Aplysin group) and administered orally with ethanol 8‐12 ml/kg.bw.d (excluding the control group), then they were administered by gavage doses of Aplysin (50, 100, 150 mg/kg.bw.d) after one hour. The rats were sacrificed after six weeks, and then liver tissue sections were utilized for pathological analysis by using light and electron microscopy. Compare with the normal group, the levels of IL‐1 (15882.3±633.8 vs 13505.5±384.6), IL‐6 (1361.1±81.1 vs 1155.4±72.4) in liver homogenate were increased significantly in ethanol‐model group by using ELISA mehtod, and with the increasing of dose of Aplysin they were both significantly decreased. The protein expression of NF‐kB and IKK were both increased significantly in ethanol‐model group by using western blot analysis, and with the increasing of dose of Aplysin they were both decreased. But the expression of protein IkB was decreased, and with the increasing of dose of Aplysin it was increased. Moreover, the mRNA expression of TNF‐αwas significantly increased in ethanol‐model group by using RT‐PCR method. These results suggested that Aplysin had a protective effect on IKK/NF/‐kB signaling pathway and related cytokines in rats exposed to ethanol. Grand Funding Source: NSFC‐31171671