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Development of Novel Probiotic and Synbiotic Mouse Diets Containing Bifidobacterium infantis , Whole Cranberry Powder and Pectin
Author(s) -
Nguyen Thuy,
Kimble Lindsey,
Mathison Bridget,
Chew Boon
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.755.10
Subject(s) - probiotic , food science , bifidobacterium , bifidobacterium longum , biology , agar , pectin , bifidobacterium breve , bacteria , microbiology and biotechnology , lactobacillus , fermentation , genetics
Current methods of feeding probiotics in animal models are limited to oral gavage, suspension in drinking water or the addition of freeze‐dried bacteria to dry food pellets; these methods may cause animal distress, loss of probiotic viability, and functionality. Feeding synbiotic diets (combinations of prebiotics and probiotics) may be more representative of human probiotic consumption. We developed a novel agar‐based diet containing live Bifidobacterium longum subsp. infantis (Bi). AIN‐93G mouse diets were formulated to provide 1‐2×10 9 cfu Bi/d and stored at 4, ‐20 and ‐80 ºC for up to 5 d. Bi dose was assessed via viable plate counts daily during storage and after 24 h at 23 ºC to mimic feeding conditions. Bi remained viable over the 5 d storage at ‐80 ºC, with concentrations from 0.95 to 1.19 ×10 9 cfu/4 g diet. Bi also remained viable after 24 h at 23 ºC with concentrations from 0.95 to 1.17 ×10 9 cfu/4 g diet. However, Bi viability decreased after 5 d at ‐20 ºC and 3 d storage at 4 ºC. The addition of 3% whole cranberry powder (WCP) and pectin had no effect ( P <0.05) on Bi viability; overall Bi dose was 1.08 ± 0.04, 1.07 ± 0.03 and 1.00 ± 0.07 ×10 9 cfu/4g diet for Bi, Bi+WCP and Bi+pectin. Furthermore, there was no difference ( P <0.05) in food intake in female BALB/c mice (6 wk old; n=48) fed AIN‐93G control diets or diets supplemented with Bi or Bi+pectin for 14 d. Mice fed WCP had higher intake ( P <0.05) than Bi and Bi+pectin diets. Overall, the mice consumed 3.73 ± 0.02 g/d and 1.48 ± 0.03 ×10 9 cfu Bi/d. We successfully developed a novel diet for feeding live probiotic and synbiotics in murine models. Supported by the ARC, Washington State University.