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Hippocampal Metabolites Correlate with Neuroimaging Outcomes in the Piglet
Author(s) -
Mudd Austin,
Alexander Lindsey,
Waworuntu Rosaline,
Berg Brian,
Donovan Sharon,
Dilger Ryan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.754.5
Subject(s) - hippocampal formation , diffusion mri , neuroimaging , fractional anisotropy , magnetic resonance imaging , metabolome , medicine , fatty acid , metabolomics , hippocampus , pathology , neuroscience , biology , radiology , bioinformatics , biochemistry , metabolite
By combining magnetic resonance imaging (MRI) and metabolomic profiling techniques, the objective of this study was to elucidate relationships between brain structure and hippocampal metabolites in the piglet. Two‐day‐old, vaginally‐delivered male piglets (n=24) were artificially reared using standardized procedures and feeding a custom milk replacer formulated to meet piglet nutrient requirements. At 30 d of age, piglets underwent MRI procedures, and brain tissue was collected 24 h post‐imaging for metabolomic and lipodomic profiling of hippocampal tissue. Analysis of MRI data in 19 brain regions yielded volumetric estimates as well as microstructural details measured by diffusion tensor fractional anisotropy (FA), and radial (RD), axial (AD), and mean (MD) diffusivities, which provide directional characterization of water movement within axons. Comparison of fatty acids in n‐3, n‐6, and n‐9 categories with MRI measures yielded correlations (P < 0.05) in 150 of 2726, 162 of 3596, and 129 of 2494 possible outcomes, respectively. Neuroimaging outcomes that were highly correlative across fatty acid categories included MD, RD, and AD in the internal capsule and right hippocampus, suggesting ongoing myelination in the piglet brain. Nervonic acid, a fatty acid known to be prevalent at peak myelination, was correlated with 26 of 58 total outcomes, further supporting the link between metabolism and neurodevelopment. In conclusion, significant correlations between metabolic and structural outcomes in the neonatal piglet brain emphasize targets whereby dietary manipulation may alter neurodevelopmental patterns. Supported by Mead Johnson Nutrition.