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Tissue Distribution of Oxidases and Transaminases mRNA in Broiler Chickens Fed with Methionine Isomers or Precursors
Author(s) -
Zhang Shuai,
Saremi Behnam,
Gilbert Elizabeth,
Wong Eric
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.754.25
Subject(s) - broiler , methionine , jejunum , transamination , aspartate transaminase , duodenum , oxidase test , ileum , transaminase , alanine transaminase , chemistry , amino acid , biochemistry , messenger rna , medicine , biology , endocrinology , enzyme , alkaline phosphatase , food science , gene
Methionine (Met) is an essential amino acid in animal diets. The common dietary supplemental Met sources include DL‐Met and the analog DL‐2‐hydroxy‐4‐methylthio butanoic acid (HMTBA). D‐Met and DL‐HMTBA must be converted to L‐Met via oxidation and transamination for utilization. We hypothesized that there are different tissue distribution and mRNA expression patterns of enzymes that convert different dietary Met sources in broiler chickens. We collected liver, muscle, duodenum, jejunum and ileum at days 10, 21 and 26 from male Cobb chickens fed a control (deficient in Met) or DL‐Met (T1), L‐Met (T2) or DL‐HMTBA (T3) supplemented diets (n=5). The mRNA level of 3 oxidases and 4 transaminases, which we considered may play a role, was measured by real‐time PCR. Data calculated using qBase PLUS were analyzed by ANOVA using JMP 10. The statistical model included the main effects of treatment, tissue, age and the interactions. Means were separated using Tukey's Test ( P <0.05). The L‐HMTBA oxidase (L‐2‐hydroxy acid oxidase) mRNA was more abundant in liver, while D‐Met oxidase (D‐amino acid oxidase) mRNA was more abundant in liver, duodenum and jejunum, as compared to other tissues. Among the 4 transaminases, branched chain amino acid transaminase (BCAT1) was the most highly expressed in muscle, and alanine‐glyoxylate aminotransferase was the most highly expressed in liver. All of the measured mRNAs had higher expression at day10 than day26. Chickens fed T1 had higher glutamic‐oxaloacetic transaminase 2 mRNA while chickens fed T3 had higher BCAT1 mRNA at day10 relative to the other treatment groups. In conclusion, the oxidases and transaminases were differentially expressed across tissues and ages in broiler chickens.