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Effect of maternal iron overload on offspring's liver injury in rats
Author(s) -
Ma Aiguo,
Cui Jing,
Jiang Ying,
Sun Yongye,
Han Xiuxia,
Cai Jing,
Wu Dayong
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.754.18
Subject(s) - medicine , offspring , endocrinology , chemistry , glutathione , liver function , immunohistochemistry , andrology , enzyme , biochemistry , pregnancy , biology , genetics
Iron overload is known to cause liver damage, however, it is not known whether this effect is transgenerational. We thus conducted a study to investigate the effect of iron overload in maternal rat on the offspring's liver function and redox status. Forty pregnant rats were randomly divided into four diet groups: high dose iron (H group, 120mg/kg·bw), middle dose (M group, 60mg/kg·bw), low dose (L group, 30mg/kg·bw), and the control (C group, 3mg/kg·bw). Iron was administered by intraperitoneal injection of 0.72ml iron dextran every other day and the entire trial lasted for 6 weeks. After 6 weeks, the serum levels of iron, and liver enzymes ALT and AST of offspring rats were determined, MDA, SOD and GSH‐PX in liver was assessed by spectrophotometry, and liver Bcl‐2 and Bax expression was measured by immunohistochemistry method. Serum iron in C group (38.34μmol/L) was significantly lower than in M and H group (58.03 μmol/L and 61.86μmol/L, p<0.05), and the liver iron showed similar results. Bcl‐2/Bax expression was significantly higher in C group (1.22) than in M and H groups (0.89 and 0.68, p <0.05). Serum ALT and AST levels were significantly lower in C group (36.56 U/g prot and 77.62U/g prot) compared to M group (69.37U/ g prot and 96.05U/g prot ) and H group (71.95 U/ g prot and 109.93U/g prot) (p <0.05). Liver MDA level was significantly lower in C group than in M and H groups (p<0.05). Moreover, liver GSH‐PX and SOD levels were significantly higher in in C group than in H group (p<0.05). In conclusion, maternal iron overload may result in excessive iron deposition, and oxidative damage to cell function in the liver of offspring rats. Supported by NSFC‐81373000