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Milk Oligosaccharides Modulate Colonic Microbiota of Formula‐Fed Piglets
Author(s) -
Jacobi Sheila,
Yatsunenko Tanya,
Berg Brian,
Chichlowski Maciej,
Odle Jack
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.754.11
Subject(s) - bacteroidetes , microbiome , biology , feces , gut flora , firmicutes , caecum , metagenomics , actinobacteria , food science , microbiology and biotechnology , bacteria , zoology , 16s ribosomal rna , immunology , medicine , bioinformatics , biochemistry , genetics , gene
Among many benefits to infant's health, human milk oligosaccharides (HMOs) serve as nutrients for the resident infant gut microbiota. Sialyllactose (SL) is an acidic HMO that has important biological functions, but its effect on modulating colonic microbiota is unknown. We previously reported that supplementation of formula with 3'‐ or 6'‐SL can enrich ganglioside SA in the brain of suckling piglets. The current study aimed to determine if different isomers of SL modulate the microbiome of developing neonatal piglets. Day‐old pigs were randomized among 6 diets (control, 3'‐SL at 2 or 4g/L, 6'‐SL at 2 or 4g/L, or 2g/L polydextrose + 2g/L galacto‐oligosaccharide; n=9 per group) and fed 3 times per day for 21 days. Pigs were euthanized and intestinal digesta were analyzed from the proximal (PC) and distal colon (DC). The microbiome analysis was performed via 16S rDNA Illumina sequencing. Dietary SL did not affect feed intake, growth or fecal consistency. Sampling location and treatment caused significant changes in the microbial taxa of the PC and DC ( P <0.05, Adonis test). There was a significant microbiome difference between control and 4 g/L 6'SL diets ( P <0.01, Adonis test). Specifically, an increase in bacterial taxa belonging to the phyla Actinobacteria and Bacteroidetes was observed ( P <0.01). In conclusion, supplementation of formula with 6'‐SL modulates gut associated microbiota of suckling piglets, and identified taxa could be the candidates for ‘next generation' probiotics enhancing the biological functions of SL. Funded by Mead Johnson Nutrition.