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Impact of Dietary Broccoli on Liver Cancer in B6C3F1 Male Mice Fed a Western Diet
Author(s) -
Chen YungJu,
Wallig Matthew,
Jeffery Elizabeth
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.753.4
Subject(s) - medicine , fatty liver , liver cancer , obesity , calorie , triglyceride , endocrinology , cancer , disease , cholesterol
Westernized eating habits, high in dietary fat and refined carbohydrate (CHO), are thought to play an important role in the increased prevalence of obesity world‐wide. Obesity and associated inflammation aggravate several chronic diseases, including non‐alcoholic fatty liver disease (NAFLD) and liver cancer. In men, obesity increases risk for liver cancer 4‐fold. We hypothesized that inclusion of broccoli in the diet may both inhibit NAFLD and lower the risk for liver cancer. We aimed to determine if long‐term dietary broccoli can inhibit liver cancer development in mice receiving a Western diet. Male B6C3F1 mice were divided into 4 groups (n=18) and fed: control (AIN‐93 M), 10% broccoli, Western (45% fat, 40.3% CHO and 14.7% protein by calories) or Western + 10% broccoli diets starting at 5 weeks of age. After 1 week, mice were treated with 45 mg/kg diethylnitrosamine (DEN; n=12/group) or saline (n=6/group) once per week for 6 weeks. Mice were killed 6 months after the last DEN treatment. Data were analyzed using 3‐way analysis of variance. Results show that the Western diet increased body weight ( P <0.0001), % liver weight ( P <0.01), plasma alanine aminotransferase (ALT; P <0.01), NAFLD score ( P <0.05), total liver triglyceride (TG; P <0.0001), visible liver nodule number ( P <0.0001) and size ( P <0.01). Inclusion of broccoli in the diet decreased plasma ALT ( P <0.0001), NAFLD score ( P <0.0001), total liver TG ( P <0.001) and visible liver nodule number ( P <0.01) in both control‐ and Western‐fed mice. We conclude that dietary broccoli protects against Western diet‐enhanced NAFLD and liver cancer. Supported by grant 5RO3CA162539 from NCI.