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Anti‐Carcinogenic Properties of Camel Milk Protein, β‐Casein, against Azoxymethane‐Induced Cancer in Rat Colon
Author(s) -
AlAlawi Ahmed,
Waly Mostafa
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.753.11
Subject(s) - azoxymethane , aberrant crypt foci , casein , glutathione , colorectal cancer , carcinogen , carcinogenesis , basal (medicine) , cancer , medicine , crypt , endocrinology , chemistry , cancer research , biochemistry , enzyme , colonic disease , insulin
Background Azoxymethane (AOM) induce colon cancer in experimental animal; in mechanism that is mediated by glutathione (GSH) depletion and impairing total antioxidant capacity (TAC) in colonic cells. Objective: This study investigated the effectiveness of, β‐Casein (extracted from camel milk) in the prevention of AOM‐induced colon cancer. Method: The experimental animals received AOM intraperitonial injection in the presence or absence of oral supplementation of β‐Casein, meanwhile the control group was fed basal diet. After 8 weeks, the rats were sacrificed and the colon tissues were examined for aberrant crypt foci (ACF) enumeration and for biochemical measurements of GSH and TAC. Results Our data show that AOM‐induced GSH depletion that was associated with an impaired TAC in colonic mucosal tissues and the ACF counts were significantly high. The concomitant treatments of AOM‐injected rats with β‐Casein reduce the AOM‐mediated carcinogenesis. Conclusion β‐Casein exhibited anti‐carcinogenic effect against AOM‐induced cancer in rat colon. In addition, β‐Casein abrogated the AOM‐mediated GSH depletion and TAC impairment.