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Effects of Black Chokeberry ( Aronia melanocarpa ) Extracts on Adhesion, Invasion and Migration in SK‐Hep1 Human Hepatoma Cancer Cells
Author(s) -
Hwang EunSun,
Do Thi Nhuan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.752.9
Subject(s) - chemistry , traditional medicine , cancer cell , microbiology and biotechnology , biochemistry , cancer , botany , biology , medicine , genetics
Black chokeberry ( Aronia melanocarpa ), a perennial, deciduous shrub belonging to the Rosacea family, has been reported to have anticancer activity, but little is known about its molecular mechanisms of action. The objective of this study was to determine the effects of black hokeberry extract on cancer cell proliferation, invasion and metastasis in SK‐Hep1 cells using migration and invasion assays. We also investigated the relationship on MMP‐2/‐9 and TIMP‐1/‐2 expression at both the protein and the gene level in SK‐Hep1 human hepatoma carcinoma cells after black chokeberry extract treatment. Black chokeberry extract inhibited SK‐Hep1 cancer cell growth in a dose‐ and time‐dependent manners, in cell numbers significantly reduced by 39.0~58.0 and 54.6~71.7 at 400mg/mL black chokeberry extracts for 24 hr and 48 hr incubation, respectively. In an invasion assay conducted in Transwell chambers, black chokeberry extract showed 24.6% and 47.2% inhibition of cancer cell at 200mg/mL and 400 mg/mL, respectively, compared to the control. The mRNA levels of both MMP‐2 and MMP‐9 were down‐regulated by black chokeberry extract treatment in a dose‐dependent manner. Black chokeberry extract at 400 mg/ml was inhibited MMP‐2 and MMP‐9 express by 64.1% and 100.0%, respectively. There was inversely related in mRNA contents between MMP‐2/‐9 and TIMP‐1/‐2 expression in SK‐Hep1 cells by black chokeberry extract treatment. Our results demonstrate the antimetastatic properties of black chokeberry in inhibiting the adhesion, invasion, and migration of SK‐Hep1 human hepatoma cancer cells.