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Walnut Phenol Extracts Inhibit Stemness of Colon Cancer Stem Cells in Vitro
Author(s) -
Kim YooSun,
Choi SangWoon,
Min Soo Jin,
Lee Jisoo,
Kim Yuri
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.752.10
Subject(s) - cd44 , cancer stem cell , colorectal cancer , cancer , cancer cell , cancer research , flow cytometry , stem cell , in vitro , mtt assay , cell , medicine , biology , immunology , biochemistry , microbiology and biotechnology
Colon cancer remains one of the major cancer types and a leading cause of cancer‐related deaths in the U.S as well as in Korea. Cancer stem cells (CSCs) are a small subpopulation of cancer cells that can self‐renew and undergo differentiation into multiple lineages which are responsible for key elements of colon cancer progression and recurrence. Therefore, CSCs appears as new targets for cancer therapeutic development. Walnuts, the seeds of Juglans regia L., are highly consumed in many countries and walnut extracts are rich in unsaturated fatty acids and polyphenolic compounds. While walnut phenolic extracts (WPE) have been previously shown to exert various anti‐oxidant and anti‐inflammatory effects, the anti‐cancer effects of WPE on colon cancer cells remains poorly understood. The aim of this study is to investigate the effects of walnut extraction on cancer stemness of colon cancer cells by suppressing self‐renewal capacity and CSC markers. To isolate colon CSCs, CD133+/CD44+ cell subsets in HT‐29 and HCT116 colon cancer cells were sorted by Flow Cytometry Cell Sorting (FACs) system. WPE treatment significantly inhibited cell survival that was measured by MTT assay. In addition, WPE suppressed colony formation and non‐adherent sphere formation which are characteristics of self‐renewal capacity of CSCs. We further tested the efficacy of WPE on CSCs markers. The result showed that WPE down‐regulated protein expression and mRNA level of CSC markers, including DLK1, CD44, and CD133. In conclusion, the present study provided the first evidence that WPE may have therapeutic potential in colon cancers targeting CSCs.