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Effects of Plasma Phospholipid Fatty Acid Composition and PPAR‐alpha L162V Polymorphism on C‐reactive Protein Levels in Response to an n‐3 Fatty Acid Supplementation
Author(s) -
Tremblay Bénédicte L,
Rudkowska Iwona,
Lemieux Simone,
Couture Patrick,
Vohl MarieClaude
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.750.10
Subject(s) - phospholipid , chemistry , fatty acid , medicine , alpha (finance) , endocrinology , biochemistry , biology , membrane , construct validity , nursing , patient satisfaction
Background Fish‐oil derived fatty acids (FAs) of the n‐3 family including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) reduce inflammation through several underlying mechanisms including altered phospholipids FA composition. The L162V polymorphism of the PPAR‐α gene is associated with a deteriorated metabolic profile and with obesity indices in numerous studies. Objective: To study whether the PPAR‐α L162V polymorphism and changes in plasma phospholipid FA composition influence plasma C‐reactive protein (CRP) levels in healthy adults following the n‐3 FA supplementation. Method: 189 subjects were supplemented daily with 3g of n‐3 FAs (1.9‐2.2g EPA and 1.1g DHA) during six weeks. Changes in CRP levels are defined by Δ CRP and changes in plasma phospholipids n‐3 (EPA+DHA) by Δ n‐3. Results: In univariate analyses, Δ n‐3 was negatively correlated with Δ CRP (r=‐0.17 p=0.02). After stratification for sex, correlations were stronger in men (r=‐0.24 p=0.02) and no longer significant in women (r=‐0.11 p=0.31). In multiple linear regression analyses including age, sex, BMI, and hormonal contraceptives as independent variables, Δ n‐3 (3.65% p= 0.01) and PPAR‐α L162V (2.10% p= 0.04) were the strongest correlates of Δ CRP. Conclusions These results suggest that the incorporation of n‐3 FAs in plasma phospholipids and PPAR‐α L162V polymorphism may contribute to changes in plasma CRP levels in response to an n‐3 FA supplementation. Funding provided by CIHR Operating Grant.