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Effect of n‐3 fatty acids and their derivatives on the expression of inflammatory genes in cultured THP1 macrophages
Author(s) -
AllamNdoul Bénédicte,
Vohl MarieClaude,
Guénard Frédéric,
Barbier Olivier
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.750.1
Subject(s) - docosahexaenoic acid , eicosapentaenoic acid , inflammation , lipopolysaccharide , tumor necrosis factor alpha , gene expression , phorbol , macrophage , chemistry , microbiology and biotechnology , gene , polyunsaturated fatty acid , fatty acid , biology , biochemistry , pharmacology , immunology , in vitro , enzyme , protein kinase c
Background Uncontrolled inflammation participates in the development of chronic inflammatory diseases. Beneficial effects of the consumption of n‐3 fatty acids (n‐3 FAs), mostly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and their derivatives (resolvins E and D) on inflammation have been reported. Objective: To study the effects of EPA, DHA and a mixture of EPA+DHA on the expression of inflammatory genes in cultured THP1 macrophages. Methods: THP1 monocytes were transformed into macrophages after an incubation of 72h into phorbol‐12‐myristate‐13‐acetate. Inflammation was triggered by lipopolysaccharide (10ng/mL during 6h and 18h). Then cells were incubated for 24h in the presence of n‐3 FAs (10, 25, 50, 100 µM). Total RNA was isolated and TNFA , IL1B , IL6 , MCP1 , NOS2 mRNA levels were measured by real‐time PCR using TaqMan technology. Results: A down‐regulation of pro‐inflammatory genes ( MCP1 , IL1B and TNFA ) and an up‐regulation of anti‐inflammatory gene MGST1 were observed. A dose‐dependent response following treatment with EPA and DHA (10, 25, 50 and 100 µM) was also observed, each n‐3 FAhaving a different effect on the expression of the genes studied. Conclusion These results suggest that in stimulated macrophages, expression levels of genes involved in inflammation are influenced by n‐3 FAs.