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A Non‐targeted Metabolomic Approach to Identify Potential Plasma Lipophilic Biomarkers of Inadequate Vitamin B 6 Status, Induced by Low B 6 Intake, Exposure to the Anti‐B 6 Factor 1‐Amino D‐proline, or Their Interaction, in a Rat Model
Author(s) -
Mayengbam Shyamchand,
House James,
Aliani Michel
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.748.4
Subject(s) - metabolomics , vitamin b , medicine , chemistry , pharmacology , vitamin , chromatography
Vitamin B 6 status in the body is affected by several factors including dietary supply of the antivitamin B 6 factor, 1‐amino D‐proline (1ADP) which is present in flaxseed. Owing to the prevalence of moderate B 6 deficiency in the general population, a co‐insult of 1ADP exposure may lead to a further deterioration of B 6 status. To this end, we applied a non‐targeted metabolomics approach to identify potential plasma lipophilic biomarkers of 1ADP injury in moderately vitamin B 6 ‐deficient rats. Male weaning rats (n=6/treatment) received a semi‐purified diet containing PN·HCl at either 7 (optimal B 6 ) or 0.7mg/kg diet (moderate B 6 ), each with 0 or 10mg/kg diet of synthetic 1ADP for 5 weeks. Plasma lipophilic metabolites were extracted in acetonitrile for analysis via LC‐QTOF/MS. Ten potential lipophilic biomarkers: glycocholic acid, glycoursodeoxycholic acid, murocholic acid , N‐docosahexaenoyl GABA, N‐arachidonoyl GABA, lumula, nandrolone, orthothymotinic acid , cystamine, and 3‐mehtyleneoxindole were identified out of >2500 detected entities. Changes in these metabolites revealed potential defects in the biosynthesis and metabolism of bile acids, N‐acyl amino acids, analgenic androgens, anti‐inflammatory and neuroprotective molecules. These data provide new insights into the impact of B 6 inadequacy on pathways linked to vitamin B 6 metabolism. Supported by the Natural Sciences and Engineering Research Council of Canada(NSERC).