Premium
Slower Rate of Fat Absorption at Dinner versus Lunch is Associated with Lower Concentrations of Triacylglycerols (TG) the Following Morning
Author(s) -
JacomeSosa Miriam,
Hu Qiong,
Manrique Camila,
Parks Elizabeth
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.744.7
Subject(s) - morning , postprandial , meal , evening , tripalmitin , chemistry , endocrinology , medicine , thermogenesis , zoology , absorption rate , absorption (acoustics) , food science , insulin , biology , obesity , biochemistry , chromatography , physics , astronomy , acoustics
High postprandial TG are a risk factor for cardiovascular disease (CVD) and little is known about the impact of successive meals on the circadian TG pattern. Here, we measured the rate of fat absorption during two sequential meals to determine how lipid processing after dinner contributes to fasting TG concentrations the next morning. Non‐diabetic adults (n=6, BMI 20‐49, fasting TG 50‐198 mg/dL) were fed a low‐fat breakfast, followed by a lunch and dinner of identical composition (35% fat, 46% carbohydrates and 18% protein). Stable isotopes were added to lunch (d 31 ‐tripalmitin) and dinner ( 13 C 4 ‐tripalmitin) to measure the kinetics of dietary fat absorption in TRL‐TG by GC/MS. Blood samples were taken intermittently throughout the day and through the next morning. For insulin and glucose, the AUCs were not significantly different between the two meals. However, the rate of fat absorption was 1.9‐fold higher after lunch than dinner (0.27±0.03 vs 0.14±0.04 mmol/L/h, respectively; P =0.04). Interestingly, faster dinner absorption rates were strongly correlated with higher 5h incremental contributions of dinner fat to TRL‐TG (r 2 =0.996; P =0.0001). These data suggest that faster absorption contributes to poor tolerance to dietary fat. Further, faster absorption correlated with subsequently higher fasting TG (r 2 =0.975; P =0.0002). In summary, isotopic labelling of successive meals allows for the identification of diurnal control of TG metabolism. These data provide a mechanism to explain why therapies that lower evening meal TG absorption rates, such as increased fiber content or reduced fat content of meals, may reduce CVD risk.