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Dietary dairy proteins improve lipoprotein profiles in C57/Bl6 mice
Author(s) -
Price Tara,
Wu Xiuzhi,
Baskaran Sangeetha,
Walzem Rosemary
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.744.1
Subject(s) - lipoprotein , weaning , cholesterol , chemistry , endocrinology , context (archaeology) , intestinal permeability , medicine , gut flora , biology , food science , biochemistry , immunology , paleontology
A third of HDL forms in the gut; HDL's Apo A1 also acts in innate immune function. Thus microbiota may alter HDL biology. Gut microbiota differed in C57/Bl6 mice fed from weaning to 13wks with diets providing 30%EN fat, 50%EN carbohydrate and 20%EN protein as either isolated soy protein (ISP), dried whole milk powder (DWMP), milk protein concentrate (MPC) or milk fat globule membrane (MFGM). Growth rates, feed intakes and final body weights were similar. Overall, dairy proteins had similar effects on lipoprotein classes. Relative to ISP, dairy proteins increased triacylglycerol‐rich lipoproteins (2.8% v.1.4%) and HDL (75.6% v. 70.0%) and decreased LDL (21.6% v. 28.5%, p<0.05) as determined by AUC for fluorescently labeled lipoproteins imaged after isopycnic separation. Small dense LDL were reduced 70% in dairy protein‐fed mice (p<0.005). HDL2a increased ~12% in mice fed MPC and MFGM (p<0.05). Cytokines and inflammatory marker genes showed a non‐inflammatory state for all groups. Gut permeability and plasma LPS concentrations were low for all groups; permeability was least (p<0.05) in DWMP fed mice. Overall gut motility was similar. Small intestinal and hepatic ApoA1 gene expression was similar among diet groups indicating that neither gene expression nor inflammation led to differences in HDL. Consumption of dairy proteins appears to improve lipoprotein profiles in C57/Bl6 mice when consumed within the context of a US Dietary Guidelines macronutrient distribution. Funding provided by National Dairy Council project #1120 administered by Dairy Research Institute.