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Effect of different types of dietary proteins on liver and muscle mTORC1 pathway after nutritional recovery
Author(s) -
RodriguezGaytran Cynthia,
MárquezMota Claudia,
Tovar Armando,
Torres Nimbe
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.742.8
Subject(s) - casein , mtorc1 , p70 s6 kinase 1 , soy protein , chemistry , phosphorylation , food science , weight gain , biochemistry , biology , medicine , endocrinology , body weight , protein kinase b
It is known that protein synthesis is regulated by mTORC1 (mechanistic target of rapamycin). When activated, mTORC1 promotes protein synthesis mainly by phosphorylation of S6K1 and 4E‐BP1. There is an interest to study some dietary proteins or dietary protein combinations for their possible use in the nutritional recovery. In the present work we studied the effect of different types and combination of dietary proteins on the mTORC1 pathway after a period of undernutrition. Sprague Dawley rats (n= 18 per group), were given a diet with a low concentration of casein (0.5% ) during 21 days in a restricted feeding period (9 hrs). After that period, rats were divided in 7 groups and were fed with one the following diets containing different protein sources: 1) 0.5% casein, 2) 20% casein, 3) 20% soy protein, 4) 20% black bean protein, 5) 20% corn protein, 6) 10% black bean‐10% corn proteins, 7) 10% soy protein‐10% corn proteins. Rats were fed with the corresponding diets during 1, 7, or 14 days. Our results showed that rats fed with casein, soy protein, soy protein‐corn or black bean‐corn had similar weight gain and recovery, meanwhile rats fed with 0.5% casein showed the lowest weight gain followed by 20% corn protein. The phosphorylation of liver and muscle mTORC1 was similar among the groups except for the groups 1 and 5. Rats fed with the combination of dietary proteins or 20% casein showed an increase in pS6K in comparison with rats fed 0.5% casein or 20% corn protein. Thus, the results indicate that the combination of dietary proteins with corn protein probably enhance protein synthesis in a similar extent than 20% casein due to the high leucine content in the corn protein through the activation of mTOR signaling pathway via S6K.