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Nonessential Amino Acids as Nitrogen Sources in Adult Men Examined Using the Indicator Amino Acid Oxidation Technique
Author(s) -
Cooper Leah,
Ball Ron,
Pencharz Paul,
Sakai Ryosei,
Elango Rajavel
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.742.11
Subject(s) - amino acid , chemistry , urine , medicine , endocrinology , biochemistry
Nutritionally, there is a dietary requirement for the essential amino acids (EAA) but also a requirement for nitrogen (N) intake for the de novo synthesis of the nonessential amino acids (NEAA). It has been suggested that some NEAA may be more metabolically important than others. The goal of this study was to determine whether among the NEAA (Ala, Arg, Asn, Asp, Gln, Glu, Gly, Pro, Ser) there was an ideal N source using the indicator amino acid oxidation (IAAO) technique. Subjects were maintained on an adaptation diet for 2 days prior to each test day. Each subject participated in 10 test diet intakes, assigned randomly. One test intake was a base diet consisting of only the EAA provided at the recommended dietary allowance. All other test intakes involved the base diet with the addition of one NEAA to meet a 50:50 ratio of EAA: NEAA on a N basis. The diets were isocaloric and isonitrogenous, given as hourly meals. Each study day followed the IAAO protocol using 13 C‐Phenylalanine as the indicator. Breath and urine samples were collected at baseline and isotopic steady state. Enrichments of 13 C in breath were analyzed by continuous‐flow isotope ratio mass spectrometry to calculate F 13 CO 2 . Repeated measures ANOVA with post hoc multiple comparisons were done on the F 13 CO 2 data. Preliminary results indicate that 7 of the 9 NEAA decrease IAAO significantly and suggest that in healthy males, most NEAA are good N sources, in the presence of adequate EAAs. (Support by Ajinomoto Co. Inc.)