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Amino Acids in Tanzanian Children with Sickle Cell Disease: Baseline results of the Vascular Function Intervention Trial (V‐FIT)
Author(s) -
Marealle Alphonce,
Makani Julie,
Kirkham Fenella,
Prentice Andrew,
Cox Sharon
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.729.14
Subject(s) - medicine , wasting , standard score , amino acid , body mass index , micronutrient , gastroenterology , zoology , biochemistry , chemistry , biology , mathematics , pathology , statistics
Poor nutritional status is common in SCD but there are no strategies to improve nutrition. Vascular function is also impaired and may underlie SCD related complications. V‐FIT (NCT01718054) is a double blind, random order, cross over trial of a daily ready‐to‐use supplementary food (RUSF) (500 kcal, 1 RDA vits & minerals, 1mg folate, Nutriset) and the same RUSF fortified with arginine & citrulline (RUSFv, 0.2g/kg/d & 0.1g/kg/d), designed to improve growth and vascular function. Here we report baseline amino acid profiles, nutritional status and their association in children enrolled in V‐FIT.Methods Children (N=119) with SCD aged 8‐11.9 y ( Fig 1 ) were enrolled. Blood samples were collected, height, weight and whole body fat free mass (WBFFM) measured by impedance (Tanita BC418). Blood samples were processed within 2h and amino acids measured (Biochrom 30). Results Stunting (height for age Z score [HAZ] <‐2) and wasting (BMI for age Z score [BMIZ] <‐2) were common: 53% & 37%. Mean HAZ = ‐2.09 (SD=1.01) & BMIZ = ‐1.71 (SD=0.88). Amino acid concentrations (μmol/L) are shown in Table 1 . Mean (+/‐ SD) Z‐scores compared to reference values are shown in Fig 2 . Associations between amino acids and degree of stunting, wasting & WBFFM by linear regression adjusted for age & sex are shown in Table 2 .Amino acid Mean 95% CI tau 64.0 60.5 ‐ 67.5 thr 111.7 105.8 ‐ 117.6 ser 117.9 113.5 ‐ 122.3 glu 31.0 29.4 ‐ 32.6 gln 590.3 574.9 ‐ 605.7 pro 182.9 172.7 – 193.0 gly 269.4 258.7 ‐ 280.1 ala 281.0 265.5 ‐ 296.4 citr 28.7 23.4 ‐ 33.9 val 146.3 140.6 ‐ 152.1 cys 44.0 41.1 – 47.0 met 26.0 24.9 ‐ 27.0 ile 44.8 42.3 ‐ 47.1 leu 87.3 83.6 ‐ 90.9 tyr 67.8 65.1 ‐ 70.4 phe 51.3 49.4 ‐ 53.1 orn 75.6 65.8 ‐ 85.5 lys 112.3 105.9 ‐ 118.7 his 41.1 39.6 ‐ 42.5 arg 85.4 72.4 ‐ 98.5 adma 0.84 0.78 ‐ 0.89Amino Acid Stunting Wasting WBFFM thr r=0.012 P<0.001 ‐ r=0.030 P<0.001 ser r=0.012 P=0.001 ‐ r=0.027 P=0.001 gly r=0.002 P=0.085 r=0.003 P=0.025 r=0.009 P=0.006 ala r=0.002 P=0.025 ‐ r=0.004 P=0.043 citr=0.066 P=0.062 cys ‐ r=0.012 P=0.05 r=0.039 P=0.020 met r=0.028 P=0.007 ‐ r=0.073 P=0.003 ile r=0.018 P=0.005 ‐ r=0.054 P<0.001 leu r=0.008 P=0.065 ‐ r=0.025 P=0.013 orn r=0.010 P=0.068 ‐ r=0.024 P=0.068 lys r=0.011 P<0.001 ‐ r=0.025 P<0.001 his r=0.028 P=0.002 ‐ r=0.068 P=0.001 arg r=0.012 P=0.028 ‐ r=0.035 P=0.009Conclusions Children with SCD display amino acid profiles significantly different from reference data. Concentrations of several amino acids are positively associated with improved nutritional status.