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Effect of GnRH on Thimet Oligopeptidase within Prostate Cancer Cells
Author(s) -
Ramirez Yesenia,
Wolfson Adele
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.728.25
Subject(s) - dihydrotestosterone , prostate cancer , endocrinology , medicine , cell growth , chemistry , gonadotropin releasing hormone , hormone , cancer cell , prostate , androgen , cancer , cancer research , biology , luteinizing hormone , biochemistry
As prostate cancer cells lose sensitivity towards androgens, the effect of gonadotropic releasing hormone (GnRH), becomes more important as a growth factor responsible for cell proliferation. The enzyme thimet oligopeptidase (TOP) has the potential to break down and thus attenuate the effects of GnRH and other peptide growth factors. Because of its ability to inactivate or modify the activity of GnRH, TOP plays an important regulatory role in steroid hormone production, either locally or by way of the hypothalamic‐pituitary‐gonadal axis, and may have implications for treatment of prostate cancer. In order to analyze the effects of TOP in the cell proliferation process, androgen‐sensitive prostate cancer cells were treated with dihydrotestosterone or GnRH. TOP activity was measured with a quenched fluorescence assay and TOP localization was determined by confocal fluorescence imaging. Quantification of TOP levels were measured via immunoblot. Results demonstrate an increase in TOP levels and activity after treatment with GnRH. These results tentatively suggest that GnRH exerts negative feedback on its own activity.