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Effect of Anti‐Cancer Reagents on the Invasiveness of Cancer Cells
Author(s) -
PARMAR NITIKA,
Garimidi Pratyusha,
Mytych Joshua,
Kennedy Shane,
Carter Katlynn
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.728.21
Subject(s) - etoposide , cell culture , cancer cell , cancer research , cancer , biology , cell , pharmacology , chemistry , medicine , chemotherapy , biochemistry , genetics
This study aimed to determine the impact of five proposed anti‐cancer agents (Berberine chloride, Azathioprine, Gossypol, Miltefosine, and Etoposide) on three female cancer cell lines: MES‐SA (uterine), C33A (cervical), and SKBR (breast). We hypothesized that treatment with these agents would decrease invasiveness based on our previous findings of their growth inhibitory properties. This was tested using the CultreCoat® 96 Well Medium BME Cell Invasion Assay. The cell lines were grown to 80‐90% confluency, then treated with the proposed anti‐cancer agents at 2µM for 24 hours. Cells were harvested and subsequently seeded in the invasion chamber for 24 hours. Cell migration, reflecting invasiveness, was monitored via a fluorimetric assay and quantified. Untreated cells served as controls. Invasiveness was influenced by the type of agent and cell line. Treatment with Gossypol increased invasiveness in all cell lines, the highest by 235% in MES‐SA. Azathioprine increased invasiveness in SKBR, and even more in C33A (141%). Berberine chloride reduced the invasiveness of SKBR by 33%, and C33A by 137%. The agent that showed the best reduction in invasiveness was Etoposide, with the biggest, 182% in C33A cells. Agents that showed an increase in invasiveness could have their potential as anti‐cancer therapy greatly reduced. Agents reducing invasiveness (particularly, Etoposide and Berberine Chloride) should be considered for further study, especially in C33A, the cell line most respondent to these agents. A dose‐dependent impact on invasiveness is currently being studied on a variety of cancer cells. Support for this project was provided by CSU Channel Island's Project Vista.