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Increase on catabolic efflux by resveratrol in Saccharomyces cerevisiae is nullified in snf1 Δ strain
Author(s) -
MadrigalPerez L A.,
MartinezOrtiz C,
CarrilloGarmendia A,
ZamudioBolaños J M.,
SotoVillagomez M A.,
PadillaPerez A K,
CornelioMartinez M I.,
GonzalezHernandez J C.,
CortesRojo C,
RamosGomez M.
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.726.15
Subject(s) - ampk , respiration , resveratrol , saccharomyces cerevisiae , catabolism , glucose transporter , chemistry , efflux , glucose uptake , biology , biochemistry , yeast , metabolism , protein kinase a , kinase , endocrinology , botany , insulin
Several studies associate resveratrol (RES) as mimetic of dietary restriction (DR), since both activate catabolic efflux (CE) by AMP protein kinase (AMPK), but not evidence exist about the activation of Snf1p (ortholog of AMPK) by RES in S. cerevisiae . Nevertheless, RES may elicit similar effects than those of DR in yeast considering that both increase replicative lifespan (RLS), and when used together a synergic patter in RLS is not observed. To further test this hypothesis, we have studied if RES increases glucose consumption (GC), respiration and growth on high‐glucose (HG), as part of the beneficial‐related mechanisms of RES seen in mammals under high‐carbohydrate and high‐fat diets. Furthermore, chronologic lifespan (CLS) at HG was tested to corroborate if RES extends CLS like DR. All the studies were performed in S. cerevisiae BY4742 (WT) and snf1Δ strain and no extension on CLS was observed with RES. Although RES‐treated cells showed an increase on growth and GC, this phenotype was not observed in snf1Δ . Moreover, WT‐RES treated cells displayed inhibition of respiration on state 4 when glucose was used as carbon source. These data correlate with observations on higher eukaryotes and suggest that RES promotes an increment on CE through Snf1p. Unexpectedly, RES inhibited respiration in WT, but this patter could be explained by the increase on NADH/NAD+ ratio enhanced by glycolysis flux which inhibits respiration at oxygened conditions, similar phenotype as the Crabtree effect. This work was funded by grant from ITSCH (3308.100310).