Premium
Specific Induction of Golgi Stress by Targeted Protein Destabilization
Author(s) -
Serebrenik Yevgeniy,
Crews Craig
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.723.5
Subject(s) - golgi apparatus , microbiology and biotechnology , stress (linguistics) , unfolded protein response , fight or flight response , chemistry , biology , endoplasmic reticulum , biochemistry , gene , linguistics , philosophy
Little is known about stress induced by increased protein load and misfolding within the Golgi apparatus. Studies performed in secretory cells requiring extensive O‐linked glycosylation reveal particularly large and well‐developed Golgi, suggesting the existence of a Golgi‐specific stress response pathway. Unfortunately, experiments to elucidate Golgi stress have been severely hampered, as there are no specific, well‐characterized inducers of Golgi stress. To overcome these limitations, we have developed a system utilizing a transcriptionally inducible Golgi apparatus‐localized protein which can be destabilized via a small molecule hydrophobic tag.We have found that targeted protein destabilization in this way is capable of inducing expression of genes known to be associated with Golgi stress. Consequently, this system will be important for detailed characterization of the Golgi stress response pathway.