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RNA‐Seq Reveals Differential hnRNP‐RNA Interactions During VSV Infection
Author(s) -
Davidson Adam,
Lyles Douglas,
Alexander Rebecca
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.718.22
Subject(s) - biology , rna , vesicular stomatitis virus , heterogeneous nuclear ribonucleoprotein , ribonucleoprotein , virology , rna virus , heterogeneous ribonucleoprotein particle , rna binding protein , microbiology and biotechnology , virus , genetics , gene
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a class of RNA‐binding proteins that carry out numerous cellular functions. hnRNPs serve as RNA shuttling proteins in healthy cells, transporting nascent RNA from the nucleus to the cytoplasm. Recent work by others has shown that hnRNP A1, hnRNP K and hnRNP C1/C2, among others, are relocalized to the cytoplasm during infection by vesicular stomatitis virus (VSV), the purpose of which is not fully understood. Vesicular Stomatitis Virus (VSV) is a non‐segmented negative strand virus of the Rhabdoviridae family. Genomic VSV is transcribed by virally encoded RNA‐dependent RNA polymerase to generate the mRNA responsible for protein synthesis in the host cell. As a weak human pathogen, it is a model organism for the more infectious viral agents influenza and rabies. VSV is an emerging player in oncolytic viriotherapy, as it is capable of tumor suppression through a variety of mechanisms including cellular hypoxia, inflammatory cytokine release and direct cell lysis. In the context of innate immunity, the interaction between hnRNPs and both viral and cellular mRNA is explored through RNA‐Seq, a deep‐sequencing approach. Each hnRNP displays a differential affinity for cellular and viral transcripts between mock‐infected and VSV‐infected states, indicating the occurrence of RNA exchange and a directed purpose for this host‐pathogen interaction.