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Mechanism of Nicotinamide Riboside as an Aid to Weight Loss
Author(s) -
Brenner Charles,
Weidemann Benjamin,
Trammell Samuel,
Coon Joshua,
Yu Liping,
Migaud Marie,
Grobe Justin
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.717.19
Subject(s) - weight loss , endocrinology , medicine , very low calorie diet , calorie restriction , insulin resistance , chemistry , biochemistry , biology , insulin , obesity
Caged mice represent an attractive model system with which to dissect the mechanistic basis for dietary and activity interventions because one can control for food amounts, dietary compositions, supplementation, and exercise in addition to genotype. Here we report that oral nicotinamide riboside (NR) supplementation increases weight loss with respect to nonsupplemented C57BL/6 mice. NR‐dependent weight loss was much more effective than resistance to weight gain on high fat diet (HFD), which has been previously reported. To assess weight loss, mice were first fattened on HFD and then assigned to normal chow plus or minus NR. Supplemented and nonsupplemented mice were also randomized to 5 days/week forced exercise or not. NR and exercise promoted weight loss individually and in an additive fashion. To assess the mechanism by which NR promotes weight loss, we measured calorie intake, activity, calorie waisted, resting metabolic rate, body composition, glucose tolerance, insulin sensitivity, and a variety of biochemical and metabolomic parameters related to liver mitochondrial function. The data are consistent with a novel malabsorptive mechanism for weight loss that can be tracked by excreted biomarkers. NR analogs and proteomic analysis of mitochondrial post‐translational modifications are being used to further characterize the mechanistic basis for NR activity in weight loss.