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Adipocyte‐Breast Cancer Cell Interactions: Preventive Effects of Omega‐3 Fatty Acids
Author(s) -
Aljawadi Arwa,
Ouertani Meriam,
Siriwardhalin,
Scoggin Shane,
Gollahon Lauren,
Dharmawardhane Surangani,
MoustaidMoussa Naima
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.715.30
Subject(s) - adipose tissue , breast cancer , cancer , endocrinology , medicine , adipocyte , cancer cell , cancer research , chemistry , biology
According to the American Cancer Society, breast cancer is the most common type of cancer and the second leading cause of death among women in the United States. Menopausal women with obesity face a higher risk of developing breast cancer. Our hypothesis is that omega‐3 fatty acid, eicosapentaenoic acid (EPA) regulates adipocyte‐secreted factors thereby modulating breast cancer cell metabolism. To test this hypothesis, we investigated the interaction between adipocytes and breast cancer cells with emphasis on the role of omega 3 fatty acids in modulating this interaction. To accomplish this, the effects of conditioned media from 3T3L1 preadipocytes/differentiated adipocytes or human mesenchymal stem cells on MCF7 breast cancer cells and vice versa were analyzed. Cells were treated with 100μM of EPA, prior to conditioned media transfer. Conditioned media from EPA‐treated adipocytes reduced interleukin‐6 (IL‐6) and monocyte chemoattractant protein‐1 (MCP‐1) secretion from MCF7 cells. To further determine whether conditioned media affects cell energy metabolism, glycolytic activity was measured using seahorse extracellular flux analyzer. Glycolysis as estimated by extracellular acidification rate was reduced in MCF7 cells exposed to conditioned media from 3T3L1 preadipocytes treated with EPA. These results suggest that adipocyte‐secreted factors modulate breast cancer cell inflammation and glucose metabolism. Importantly, we identified that the anti‐inflammatory nutrient, EPA could be used for a preventive approach in adipocyte‐breast cancer cell interactions. Preventive effects of EPA in post‐menopausal obesity‐associated breast cancer merits further investigation.