Premium
Profiling of Lipids Expression Along the Rostral and Caudal Axis After Spinal Cord Injury: Matrix Assisted Laser Desorption/Ionization Approach
Author(s) -
Adam Amna,
Salzet Michel,
Devaux Stephanie
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.715.21
Subject(s) - spinal cord , white matter , chemistry , spinal cord injury , lipid metabolism , anatomy , biology , biochemistry , medicine , neuroscience , magnetic resonance imaging , radiology
Spinal cord injury (SCI) causes alteration in lipid composition and migration of lipids between the gray and white matter regions of spinal cord. Studying the prominent and region‐specific lipids in injured spinal cords can possibly lead to clinical understanding of the specific role each class of lipid plays in the inflammation process after SCI. Utilization of Matrix Assisted Laser Desorption/Ionization (MALDI)‐TOF/TOF coupled with FlexImaging and ClinProTools software for statistical analysis was imployed as a method to identify lipids that demonstrate interesting behavior post SCI. Furthermore, shotgun analysis using Q Exactive performed to possibly characterize interesting lipids. Using MALDI‐Mass Spectrometry, the region‐specificity of lipid expression and alteration in injured spinal cord were identified with progression of time. Rat tissues from 3, 7, 10 days post SCI were imaged in both negative and positive mode and revealed that visualization of region‐specific lipid is best carried in the positive reflector mode. Shotgun analysis using Orbitrap allowed for approximate characterization of the lipids with m/z ratio identified using MALDI imaging. Taking all together the data suggests that in the positive reflector mode, there is a difference in the expression in rostral and caudal region, and glycerophospholipids is possibly the most prominent class of lipid in both gray and white matter region of spinal cord post SCI.