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Hsp90, Hsp60 And HSF‐1 Genes Expression in Muscle, Heart and Brain of Thermally Manipulated Broiler Chicken
Author(s) -
AlZghoul Mohammad
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.713.2
Subject(s) - broiler , hatching , medicine , endocrinology , heat shock protein , biology , zoology , messenger rna , hormone , hsp60 , gene , hsp70 , biochemistry
The effect of thermal manipulation (TM) was investigated during embryonic days (ED) 12‐18 on the hatchability, body temperature (T b ) and on thyroid hormones (T 3 & T 4 ) and on mRNA levels of heat shock proteins (Hsp90, Hsp60 and HSF‐1) in muscle, heart and brain tissues and during thermal challenge (TC) at days 10 and 28 post‐hatching. One thousand five hundred fertile chicken eggs were randomly allocated into four groups: Control group (37.8°C), TM 1 (39°C for 9h), TM 2 (39°C for 12h) and TM 3 (39°C for 18h). Chicks from each treatment group were then randomly sub‐divided into 2 treatment groups (Naive and TC). At days 10 and 28 of age, chicks in TC groups were subjected to thermal challenge (TC) at 43.0°C for 6h while naïve chicks were kept under regular conditions. Chick's response to TC was evaluated by determination of body temperature (T b ), plasma T 3 and T 4 levels and mRNA levels of Hsp90, Hsp60 and HSF‐1 in muscle, heart and brain tissues. TM adversely affected chick's hatchability rate. The T b of TM chicks decreased compared to the control group during the first 2 weeks of age. Interestingly, during TC at days 10 and 28 of age, the T b of TM chicks was significantly lower than those of the controls. When compared to the control, TM resulted in a significant increase in mRNA levels of Hsp90, Hsp60 and HSF‐1in muscle, heart and brain tissues during embryogenesis and during TC at days 10 and 28 post‐hatching. Results of this study indicated a long‐term enhancement of Hsp90, Hsp60 and HSF‐1 gene expressions associated with improved thermotolerance acquisition in thermally challenged chicks without adversely affecting body performance parameters.