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The role of miR‐450a in oral squamous cell carcinoma carcinogenesis
Author(s) -
Hsing EnWei,
Shiah ShineGwo,
Hsiao JennRen,
Lyu PingChiang,
Chang JangYang
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.711.22
Subject(s) - biology , gene , cell , carcinogenesis , microbiology and biotechnology , genetics
microRNAs in tumor progression are crucial in head & neck cancer. Our research group found that miR‐450a were significantly higher in 46 clinical OSCC specimens than those in corresponding adjacent non‐cancerous specimens (p<0.001). However, its role in oral carcinoma has not been elucidated yet. miR‐450a is highly conserved among mammals. In homo sapiens , duplicated miR‐450a were located at adjacent loci in X chromosome. Gene expression microarray was performed to identify potential miR‐450a mediating genes. Differential gene expressions in pre‐miR‐450a‐transfected DOK and SCC15 were analyzed. Using Gene Set Enrichment Analysis, 16878 & 17000 down‐regulated genes were clarified in DOK & SCC15, respectively. To narrow down these miR‐450a targeting genes, two gene lists were independently overlapped with target predicted program microRNA.org. These two gene lists were further reduced to 419 genes in DOK & 465 genes in SCC15, and 256 genes were common in both two gene sets. We used RT‐PCR to validate a suspected membrane protein, TMEM182. The expression of TMEM182 was down‐regulated in RNA and protein levels. We verified that this TMEM182 down‐regulation was under a post‐translational level mediated by miR‐450a. Up‐regulation of miR‐450a in OSCC would enhance cell detachment ability. But, the cell proliferation was higher in miR‐450a overexpressed cells. We suggested that miR‐450a regulates cell adhesion through down‐regulation of TMEM182, and further influences cell migration in oral cancer.