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Cellular Identification of Doublecortin Positive Immature Neurons in Monkey Cortex
Author(s) -
Heyworth Nadine,
Calderazzo Samantha,
Kyada Margee,
Rosene Douglas
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.706.7
Subject(s) - doublecortin , parvalbumin , calretinin , neuroscience , neurogenesis , calbindin , biology , subventricular zone , gabaergic , subgranular zone , neocortex , cortex (anatomy) , inferior colliculus , corticogenesis , hippocampus , dentate gyrus , inhibitory postsynaptic potential , microbiology and biotechnology , neural stem cell , immunohistochemistry , stem cell , progenitor cell , nucleus , immunology
Doublecortin (DCX) is a microtubule‐associated protein expressed transiently during the maturation and migration of newborn neurons. In the adult brain, two neurogenic regions including the Subventricular Zone (SVZ) and the Subgranular Zone (SGZ) continue to produce adult generated neurons (aGN) throughout adulthood. The transient expression of DCX has also been found in various neocortical and allocortical regions in the adult brain of the rodent, rabbit, cat, monkey and human. These regions include the piriform cortex, medial prefrontal cortex, entorhinal cortex, as well as neocortical areas of the temporal lobe and insula. DCX+ cells are reported to localize mainly in superficial layers of cortex (II/III) with scattered DCX cells in deeper cortical layers. Some studies have reported these cells differentiate into excitatory neurons while others report a GABAergic phenotype. In addition to cellular identity, the origin of these neurons remains unresolved with one possibility that these cells are derived postnatally from the SVZ while others contend that they are generated prenatally. To address these issues, tissue was obtained from rhesus monkeys injected with BrdU to label aGN. Sections were processed with immunohistochemistry and confocal microscopy to determine if DCX cells in the temporal lobe co‐localize with BrdU and/or with markers of inhibitory interneurons including Calbindin, Parvalbumin, and Calretinin, or with the excitatory neuron marker CaMKII. Results show no DCX+ cells co‐localized with BrdU, indicating that DCX+ immature neurons are not adult generated and hence are leftover from development. Preliminary analysis of co‐localization with DCX shows that at least some co‐localize with GABergic markers of interneurons.