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Technical approach for visualization of the arterial and venous brain vasculature
Author(s) -
Tonnelet Romain,
Perez Manuela,
Krebs Marine,
Arnoux JeanMichel,
Labrousse Marc,
Braun Marc
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.706.2
Subject(s) - medicine , visualization , biomedical engineering , radiology , sagittal plane , superior sagittal sinus , anatomy , nuclear medicine , computer science , surgery , artificial intelligence , thrombosis
We present an original method for post‐mortem study of macro‐ and micro‐vascular of the arterial et venous bed of the brain based on 3D angiographic acquisition and micro‐radiography. Material & Method Six brains were used for this study collecting by the Anatomy Laboratory of Université de Lorraine. For arterial preparation, removal of the brain was carried out at early stage. No formalin solution was used before injection, the latter being performed as soon as the brain was removed and injected with a mixture gelatin‐barium.Injections were made using soft tipped rubber trocars introduced in the two carotids and vertebrals arteries. The same protocol were used for the venous preparation, but the two internal jugular veins were cathterized and injected. Angiographic acquisition were done on C‐arm Flat‐Panel for each specimens (isotropic resolution ‹ 200 microns). Then brains were fixed for two months in a 4% Formaldehyde solution. They were sliced in sagittal or frontal plane. Finally micro‐radiographs were done on each sections by use of a Mammograph (pixel resolution ∼ 85 microns). Results We obtained 3D volumic acquisition of the whole brain , and macro‐vascular pattern was studied on a Advantage Workstation for multiplanar and 3D‐Volume‐Rendering visualization. Micro‐radiographics acquisition showed us the micro‐vascular pattern, especially the transcerebral arteries or veins in cortico‐sub‐corticals and periventricular areas. Conclusion We used the same technique as described by Salamon and Theron for study of the brain vasculature by used of modern radiographics materials allowing a sub‐millimetric visualization of brain vessels.