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ProBDNF Negatively Stimulates the Neuronal Precursor Cells Differentiate into Mature Neurons in Rat DRG
Author(s) -
wei Ma,
jinwei Yang,
jin Ru,
tao Luo,
dongyan Wang,
xingtong Li,
tongtong Wang,
liyan Li,
jianhui Guo
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.704.6
Subject(s) - dorsal root ganglion , neuroscience , hippocampal formation , cerebellum , neurotrophin , neurotrophic factors , hippocampus , central nervous system , biology , chemistry , microbiology and biotechnology , receptor , spinal cord , biochemistry
Brain‐derived neurotrophic factor precusor (proBDNF) is present in many regions of the central nerve system (CNS) and is released in cultured cortical neurons and at hippocampal aynapses. ProBDNF can induce cell apoptosis and has inhibitory effects on neuronal events, its action mode and pathway different from mature BDNF, which lead to the survival effects. Most of the research carried out on proBDNF has concentrated on the brain, cerebellum, cortex, and hippocampus.However, the roles of proBDNF in peripheral nervous system (PNS) have not yet been reported. Our research successfully separated and purified the satellite glial cells (SGCs) from normal rat dorsal root ganglion (DRG), and labelled neurons and glial cells using Immunofluorescence. GS + , GFAP + and Tuj1 ‐ cells were detected in control groups, but GS + and Tuj1 + cells were found in groups induced by proBDNF antibody. We conclude that proBDNF may participate in the regulatory process of neuronal precursor cells differentiated into mature neurons in rat DRG. *Corresponding authors: Li‐yan Li (kmliyanl@163.com) and Jian‐hui Guo (guojianhuikm@163.com)