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Thyroid Hormone Receptor Regulation of Tooth Development in Newts
Author(s) -
Miwa Yoko,
Sunohara Masataka,
Sato Iwao
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.702.8
Subject(s) - thyroid , propylthiouracil , thyroid hormone receptor , hormone , endocrinology , medicine , hormone receptor , dental alveolus , receptor , dentistry , cancer , breast cancer
Objective Thyroid hormone receptor and thyroid hormone are contribute to the development of the teeth and the regeneration of alveolar bone following amputation in newts. However, the relationship between the mRNA transcript levels of thyroid hormone receptor in alveolar bone is unknown in blocking the effect of thyroid hormone. Method: Propylthiouracil (PTU) is a thiouracil‐derived drug used to blocking the effect of thyroid hormone. Japanese newts ( Cynops pyrrhogaster ) were maintained in aqueous solutions with or without PTU for one month and tracked the sequential development of the tooth cap, the tooth bud, and ultimately the maturation of the sequential tooth germ. Fifty days after the amputation procedure of the right mandible alveolar bone, the newts were observed the effect of PTU by the expression levels of mRNA of thyroid hormone receptor detected in‐situ hybridization and real time RT‐PCR. Result: The amputated mandible sites were observed microscopically. The level of mineralized area of alveolar bone in amputation with PTU treatment was significantly smaller than that in the amputation in blocking the effect of thyroid hormone. The expression levels of mRNA of thyroid hormone receptor was significantly increased in regenerated area of alveolar bone by real time RT‐PCR(Student's t‐test, p<0.001). Conclusion Our data suggests that thyroid horme may affect the tooth germ development and the regeneration of alveolar bone in newts.