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The flavonol quercetin modulates the chemical barrier of innate immunity in endometrium
Author(s) -
Deachapunya Chatsri,
Poonyachoti Sutthasinee
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.684.13
Subject(s) - secretion , quercetin , flavonoid , innate immune system , chemistry , western blot , biology , medicine , biochemistry , immunology , immune system , gene , antioxidant
Epithelial cells lining the uterine cavity provide both physical and chemical barrier to pathogens invading into the body system. Quercetin, the most abundant dietary flavonoid, shows the promotive and protective effects on intestinal tight junction barrier; however, information regarding its effect on chemical barrier function as secretions of fluid and anti‐microbial peptide β‐defensin in endometrium. Therefore, the effects of the flavonoid quercetin on electrolyte secretion and expression and secretion of β‐defensin (BD)‐2 were investigated in immortalized endometrial epithelial cells (PEG). Using Ussing chamber techniques, quercetin (1, 10 and 100 mM) showed the concentration‐dependent increase in Cl ‐ secretion. Parallel to the Cl ‐ secretion, high concentration of quercetin acutely stimulates BD‐2 secretion. Treatment of filter‐grown PEG cells with quercetin for 48 h was found to increase the synthesis of BD‐2 protein by Western blot analysis. The increased BD‐2 by quercetin was related to the secretion of BD‐2 into the luminal, but not basolateral compartment as assessed by ELISA. Although the significant role of uterine fluid secretion in innate immunity remains to be determined, our results indicate the potential role of quercetin on promotion of chemical barrier against microbial infections in endometrium.